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At the end of drug exposure, medium was removed and a stock solution of the green-fluorescent CyQUANT GR dye (prepared according to manufacturer's instructions) was added.
After the exposure period, 100 μl assay buffer (mixed according to suppliers' instructions) was added to the control wells.
However, adequate health literacy had no significant association with glycemic control after better understanding of health education and instructions was added to the regression model (model 2).
After air-drying, a sinapinic acid solution (70% acetonitrile, 0.1% trifluoroacetic acid) (SPA, Ciphergen Biosystems), prepared according to the manufacturer's instructions, was added to each spot.
For the positive control, 25 μl of the provided HepG2 Cell Lysate (unstimulated), prepared as per manufacturer's instructions, was added to wells.
The detection antibody (100 μl) (mixed according to suppliers instructions) was added to each sample, except to the blank, and the plate was sealed for 1 h.
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Doing so requires extra hardware to store the processor state for each thread, and when instructions are added to the queue, they must be labelled as coming from a particular thread.
Therefore, interviewer instructions were added to stimulate more meaningful responses, particularly for English language participants.
If custom instructions are added and removed from the subset one by one, then the common supergraph can be updated appropriately by applying the heuristic each time a new instruction is added.
Subsequently, fluorescently labelled conjugated mAbs (concentrations according to manufacturer's instructions) were added and incubated for 30 min at 4°C.
Optional instructions were added before the sexual items.
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CEO of Professional Science Editing for Scientists @ prosciediting.com