The optimization of the signal was performed daily for all pinhole apertures through mirror alignment, following the manufacturer's instructions, using a rhodamine standard (or other adequate reference).
The approach involves analysis and testing of existing safety and emergency regulations and personnel instructions using a specific simulation software, and further correction of the regulations and instructions basing on the results of the analysis.
The purified protein was quantified by the bicinchoninic acid assay (BCA assay) (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer's instructions using a Spectronic 20D+ spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).
Instructions Using a 3″ (7.5 cm) biscuit cutter, cut the center out of each piece of bread.
3. Follow the zip bag instructions, using a 50/50 blend of canola oil and olive oil to submerge the steak, thyme and garlic.
The mix was used according to the manufacturer's instructions using a 20 µl final volume.
Total HGF ELISA assay was performed as per manufacturer's instructions using a kit (RayBiotech, Norcross, GA).
RNA samples were reverse transcribed with RevertAid M-MuLV (Fermentas) according to manufacturer's instructions using a d(T 20 primer.
Total c-MET ELISA assay was performed as per manufacturer's instructions using a kit (R&D systems, Minneapolis, MN).
Protein expression and purification was performed according to the manufacturer's instructions, using a 36-hour self-cleavage reaction.
Luciferase activity was analyzed according to the manufacturer's instructions using a Dual Luciferase reporter assay (Promega Madison, WI).
