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After a Fifa passport inspection was completed without incident, Ramirez performed his last-minute instructions using those cups, water bottles and oranges.
Total RNA was isolated following manufacturer's instructions using RNAeasy kit (Qiagen).
At specific time points, cytotoxicity and viability were measured as per manufacturer's instructions using the MultiTox-Fluor Multiplex Cytotoxicity Assay.
PCR and extension reactions were performed in a 96-well plate according to the manufacturer's instructions, using Sequenom reagents.
Ampliflu Red stock solutions are prepared according to the manufacturer's instructions using 50-mM PBS buffer (pH 7.4).
Assays were performed according to the manufacturer's instructions using Flow Cytometer FC-500 (Beckman Coulter, Miami, FL, USA).
"From there, I can generate tool-paths (or machine cutting instructions) using computer-aided manufacturing software.
Instructions Using a 3″ (7.5 cm) biscuit cutter, cut the center out of each piece of bread.
siRNA transfections were performed as per manufacturer's instructions using Dharmafect 2 and siRNA from Dharmacon.
The mix was used according to the manufacturer's instructions using a 20 µl final volume.
DNA was extracted according to the manufacturer's instructions using the DNA Blood and Tissue Kit (Qiagen).
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CEO of Professional Science Editing for Scientists @ prosciediting.com