Utilizing current research on CUREs and on research internships, we identify and describe five dimensions of CURE instruction: use of science practices, discovery, broader relevance or importance, iteration, and collaboration.
Shannon pointed out that the length of the instructions used to encode a string of characters corresponds to the disorder, or "entropy", of that string.
Transfections were performed as per the manufacturer's instructions, using a concentration of 50 nM of siRNA.
Transfections were carried out using FuGENE 6 (Roche Applied Bioscience), following the manufacturer's instructions (using 2 µg of plasmid and 6 µl of transfection reagent).
First-strand cDNA synthesis was performed using 1 µg of total RNA with Superscript II (Invitrogen) following the manufacturer's instructions, using 500 ng of random hexamers.
The guidance also says that instructions for use of sky lanterns, under a industry-written code of practice and endorsed by ministers and trading standards officials, should include prosecution warnings.
The aids were provision of an assembly jig, instructions on use of the jig and components set up in an ergonomically-designed workplace.
Instructions regarding use of a food scale, meal plans, and 3-d food records were provided.
