Sentence examples for instructions treated with from inspiring English sources
Total RNA extracts were prepared with Trizol Reagent (Invitrogen) according to the manufacturer's instructions, treated with DNAse I then reverse transcribed with iScript cDNA synthesis kit (Biorad).
Total RNA was isolated according to the manufacturer's instructions, treated with DNase and immediately reverse transcribed by means of random hexamer primers (Roche) and Superscript II RT (Invitrogen, Life Technologies).
Total RNA was extracted from specimens at 7, 15 and 30 days using TRI Reagent (Sigma, Milan, Italy), following the manufacturer's instructions, treated with DNase (Promega) to exclude DNA contamination and stored at −80°C until the assays.
The following day, cells were transfected with indicated cDNA plasmids using lipofectamine 2000 (Invitrogen) according to the manufacturer's instructions, treated with 10 nM DHT (Wako; dissolved in ethanol) using DMEM supplemented with charcoal:dextran stripped FBS (Gemini Bio-Products), and cultured until analyzed.
After ProteinaseK treatment 500 μl of Trizol LS (Qiagen, Venlo, Limburg) was added to each sample, vortexed for 10 s, and incubated at 25°C for 5 min. Total RNA was recovered from each sample with the miRNeasy kit (Qiagen, Venlo, Limburg) following the manufacturer's instructions, treated with TURBO-DNAfree (Ambion, Carlsbad, CA), and repurified with the miRNeasy kit.
Total RNA was extracted from each sample using the Trizol reagents (Invitrogen) according to the manufacturer's instructions and treated with DNase I (Promega, Madison, WI).
Total RNA was extracted from developing karyopses using TRIzol reagent (Invitrogen, Tokyo) and the manufacturer's instructions, then treated with RNase-free DNase-I (Roche Diagnostics, Mannheim, Germany) to remove DNA contamination.
Samples were purified with the RNeasy Mini kit (Qiagen) according to the manufacturer's instructions and treated with DNase I (RNase-free DNase Set, Qiagen).
For primary human breast carcinomas, total RNA was extracted using Trizol reagent (Life Technologies) following the manufacturer's instructions and treated with DNaseI (Qiagen).
Total RNA was isolated from the dissected tissues using TRIzol Reagent (Invitrogen) according to the manufacturer's instructions and treated with DNase I (RNase-Free) (Ambion).
Total RNA was extracted using the RNeasy Mini Kit according to the manufacturer's instructions and treated with RNase-free DNase I (Qiagen).
