Sentence examples for instructions total from inspiring English sources
Nuclear proteins were extracted from 1,5×107 T-cells using the Active Motif Nuclear Extract kit according to the manufacturer's instructions, total protein concentrations of the lysates were determined by Bradford assay (Bio-Rad).
According to the manufacturer's instructions, total RNA was precipitated using the RNeasy Protect Mini Kit (Qiagen, Crawley, UK).
In accordance with the manufacturer's instructions, total RNA was isolated with TRIzol (Invitrogen, Carlsbad, CA, USA) from each sample.
Supernatants were collected and MMP-1 concentrations were determined by ELISA according to the manufacturer's instructions (Total MMP1Biotrak; Amersham Biosciences, Piscataway, NJ, USA) [ 22].
Following the manufacturer's instructions, total RNA was extracted from whole ovaries by TRIzol (Invitrogen, Carlsbad, CA, USA) and an miRNA easy minikit (Qiagen, Valencia, CA, USA).
According to manufacturer's instructions, total RNA was extracted using RNAfast 200 Kit (Fastagen) and then reversed transcribed to cDNA using PrimeScript RT Reagent Kit (Takara).
According to the manufacturer's instruction, total RNA was extracted by using TRIzol® Reagent (Invitrogen, California, USA), treated with DNase I (Invitrogen, California, USA), dissolved in RNase-free water, and stored in a − 80 °C freezer.
Total RNA was isolated from the liver tissue using a commercial kit according to the manufacturer's instruction (Total RNA and protein isolation kit, Macherey Nagel, Düren, Germany).
Total RNA was isolated with TRIzol (Invitrogen) according to the manufacturer's instructions and total mRNA was then purified using oligo (dT) magnetic beads.
Total cellular RNA was extracted using the mirVana miRNA Isolation kit (Ambion) per manufacturer's instructions for total RNA isolation.
Protein concentration was measured using a BCA Protein Assay Kit Thermo Scientificc) according to the manufacturer's instructions and total cellular glutathione was normalized to the protein content of the sample before calculating the total glutathione content compared to non-NAC treated animals.
