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The reason for the confusion was that he had married one of his gift subscriptions & the computer had not heeded his instructions to "reverse the two top names".
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For the analysis of mature miRNAs, precursor miRNA and mRNA from the same reverse transcription reaction total RNA was first treated with DNase using the TURBO DNA-free kit (Life Technologies) according to the manufacturer's instructions prior to reverse transcription.
Total RNA (1 μg), obtained using RNAprep pure tissue kit according to the manufacturer's instructions, was subjected to reverse transcription with an oligo-dT18 primer, recombinant RNAsin, and M-MLV reverse transcriptase (all Fermentas, EU).
The attacker can use the traces to reverse engineer instruction sequences when sufficient knowledge of a processor's architecture is known, thus giving clues about what the cryptosystem is processing [10, 11].
One of his first acts as Attorney General was to reverse Obama-era instructions to federal prosecutors that discouraged the pursuit of charges with mandatory minimums of prison time.
Reverse transcription (RT) was performed according to reverse transcription kit instructions to synthesize first-strand cDNA.
When Masha wants Spike to get dressed again, he takes her instructions to "do a reverse strip tease" literally.
Replace and reverse instructions to put all back together.
For RT-PCR, total RNAs were extracted from each sample using the PureLink™ Micro-to-Midi system (Invitrogen) according to the manufacturer's instructions, and reverse transcription was to generate cDNAs using PrimeScript™ RT Reagent Kit (TaKaRa).
If you are left-handed, reverse the instructions to fit.
Simply reverse these instructions to put everything else back together.
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