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This kit was used, per the manufacturer's instructions, to extract the RNA.
We also provide an online tool and instructions to extract MCS-SNPs for any region of human DNA, via the UCSC genome browser.
The Gentra Puregene Cell kit (Qiagen, Valencia, CA) was used according to the manufacturer's instructions to extract the DNA from 300 ul of the cell suspension.
We used the DNeasy Blood and Tissue Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions to extract genomic DNA from a portion of the spleen into a final elution volume of 100 µL.
We used the Maxwell 16 Viral Total Nucleic Acid Purification Kit with the Maxwell 16 instrument (Promega, Madison, WI, USA) according to the manufacturer's instructions to extract nucleic acids from the serum samples.
We used a TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions to extract RNA from the muscle biopsies and the PAXgene Blood RNA Kit (Qiagen, Hilden, Germany) to extract RNA from the blood.
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GPs received tailored written instructions how to extract a list of patients with CHF from their electronic medical record system (EMR).
Using the DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany) we followed the instructions given to extract DNA from animal tissues and used approximately 1 × 1 cm of the body wall from one individual.
In the current day wide-issue processors, the size of the instruction scheduling window (also called Issue Queue (IQ)) is limited mainly by the hardware complexity to design the logic, and thus limits the number of instructions scanned every cycle to extract instruction level parallelism (ILP).
Some PPS users may not read the instructions carefully enough to extract this intent.
The manufacturer's instructions were followed to extract viral RNA QIAGEN QIAamp Viral RNA Mini Kitss EZ1 Virus Mini Kit v2.0, catalog number 955134, Germany).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com