Sentence examples for instructions to detect the from inspiring English sources
Horseradish peroxidase-conjugated streptavidin was used according to the manufacturer's instructions to detect the transferred DNA.
The ESC supernatant was assayed by ELISA according to the manufacturer's instructions, to detect the levels of secreted TECK.
After the incubation cells were fixed and stained for EdU, according to the manufacturer's instructions to detect the number of cells in cycle during the EdU treatment.
LDH cytotoxicity assay kit II (Abcam, Cambridge, United Kingdom) was used according to manufacturer's instructions to detect the release of the cytosolic enzyme, LDH, from U937 cells following treatment with defensins.
Proteome Profiler Human Angiogenesis Array Kits (R&D Systems, Minneapolis, MN) were used per the manufacturer's instructions to detect the relative expression levels of 55 angiogenesis-related proteins in conditioned media-treated HUVECs and MV-treated mice bearing intracranial D283med-luc tumors.
An M.O.M. ImmPRESS HRP (Peroxidase) Polymer Kit (MP-2400; Vector Laboratories, Burlingame, CA, USA) and cyanine 3 conjugated goat anti-HRP (123-165-021, 1:200 dilution; Jackson ImmunoResearch Laboratories, West Grove, PA, USA) were used according to the manufacturers' instructions to detect the anti-p63 antibody.
Fluorescent TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) assays (Roche Diagnostic) were performed according to the manufacturer's instructions to detect apoptosis in tumours of the different therapeutic groups.
DyLight™ 488 rat monoclonal anti-mouse CD31 (PECAM-1) antibody (Novus Biologicals Canada ULC, Oakville ON L6M 2V5, Canada) was used in immunohistochemistry at a 1 300 dilution according to the manufacturer's instructions to detect mouse CD31+ (PECAM-1) endothelial cells in the paraffin-embedded xenogafts of A2780 ovarian tumors.
In some experiments, the Annexin V-FITC Apoptosis Detection Kit (BD-Bioscience Pharmingen, San Diego, CA, USA) was used, according to the manufacturer's instructions, to detect apoptosis.
We used the ProSpect Rotavirus R240396 (Oxoid, Kamstrupvej, Denmark) enzyme immunoassay kit, according to the manufacturer's instructions, to detect group A human ROTAV in fecal specimens.
