Sentence examples for instructions to detect from inspiring English sources
DyLight™ 488 rat monoclonal anti-mouse CD31 (PECAM-1) antibody (Novus Biologicals Canada ULC, Oakville ON L6M 2V5, Canada) was used in immunohistochemistry at a 1 300 dilution according to the manufacturer's instructions to detect mouse CD31+ (PECAM-1) endothelial cells in the paraffin-embedded xenogafts of A2780 ovarian tumors.
MTT assay (Sigma) was performed following the manufacturer's instructions to detect cell proliferation.
Horseradish peroxidase-conjugated streptavidin was used according to the manufacturer's instructions to detect the transferred DNA.
The ESC supernatant was assayed by ELISA according to the manufacturer's instructions, to detect the levels of secreted TECK.
HC2 HPV test (Digene, Gaithersburg, MD) was performed according to the manufacturer's instructions to detect HPV, starting from 4 mL of liquid cytology specimens.
After the incubation cells were fixed and stained for EdU, according to the manufacturer's instructions to detect the number of cells in cycle during the EdU treatment.
Sections were processed using a modification of the Perl's staining instruction to detect ferric iron (Bishop and Robinson, 2001; Moos and Mollgard, 1993).
A Procarta cytokine profiling kit (Panomics, Santa Clara, CA, USA) was used according to the manufacturer's instructions to simultaneously detect 12 different human chemokines and cytokines in cell culture supernatants.
The Human Phosopho-Receptor TyR&Dine KinaSystemsy Kit (R&D Systems, Wiesbaden, Germany) was used according to the manufacturer's instructions to simultaneously detect the relative tyrosine phosphorylation levels of 42 different receptor tyrosine kinases (RTKs), as described previously in more detail (Ball et al, 2007).
The senescence-associated β-galactosidase staining kit from Cell signaling technology (Beverly, MA, USA) was used according to the manufacturer's instructions to histochemically detect β-galactosidase activity at pH 6.
The Taqman miRNA assay system (Applied Biosystems, Foster City, CA) was used according to the manufacturer's instructions to quantitatively detect the expression of mature miRNAs was performed using the TaqMan miRNA Assay Kit (Applied Biosystems) containing TaqMan primers in a universal PCR master mix.
