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Then follow the instructions supplied with the kit for cutting the foam to fit around the inside of the tank and attaching it with the adhesive.
Recombinant DNA techniques were performed by using established methods [46] or following the instructions supplied with specific reagents.
Supernatants containing the recombinant proteins IL-20 and IL-24 were affinity-purified and further concentrated using anti-FLAG M2 affinity gel (Sigma) according to instructions supplied with the product.
Stomach sections were permeabilized with proteinase K, and the 3'-OH ends of the DNA fragments were then stained following the instructions supplied with the kit.
The purified small RNA was then used to prepare a sequencing library according to the instructions supplied with the TruSeq small RNA library kit (Illumina).
The HRP reaction was performed with 3-amino-9-ethyl-carbazole and hydrogen peroxide according to the instructions supplied with the streptavidin-biotin staining Vectastatin Elite Kit (Vectors Laboratories, Burlingame, CA).
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Intracellular content of GSH was measured as described by instruction supplied with the GSH and GSSG Assay Kit.
All of the 168 isolates were characterized by spoligotyping as previously described by Kamerbeek et al. [ 12] following the instruction supplied with the spoligotyping kit (Ocimum Biosolutions Company, Ijsselstein, The Netherlands).
Cycle sequencing was performed following the instructions supplied along with BigDye® Terminator v3.1 Cycle Sequencing Kit.
Colour was developed with chemiluminescence reagents according to the instructions supplied by the manufacturer (DuPont NEN, Boston, MA, USA).
Color was developed with chemiluminescence reagents according to the instructions supplied by the manufacturer (DuPont NEN, Boston, MA, USA).
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