(iii) Using the transmitter manufacturer's measurement data submitted to the FCC for type acceptance and as shown in the instruction book supplied to the licensee.
He finally agreed that the premiere could take place at the Metropolitan Opera in New York, without his being there, on the basis of performing instructions which he supplied to the conductor.
When working on the song, and while following numerous instructions that were supplied to him by West, DJ Premier took seven different styles of scratches, including drum breaks, cut them up into different rhythms, and scattered them all throughout the track, thus providing West with several different ideas for him to choose from.
At the beginning of the study an instruction sheet was supplied to the parent/carer(s) for use of the sprays.
Cells were transfected with 2 µg each DNA construct using Lipofectamine2000 (Invitrogen), according to instructions supplied by the manufacturer.
Transcriptional start sites of the GATA4 gene were mapped by rapid amplification of cDNA ends (5' RACE) using the GeneRacer kit according to instructions supplied by the manufacturer (Invitrogen).
Supernatants containing the recombinant proteins IL-20 and IL-24 were affinity-purified and further concentrated using anti-FLAG M2 affinity gel (Sigma) according to instructions supplied with the product.
Flt-1 siRNA (sc-29319), PGF siRNA (sc-44027) and control siRNA-A (sc-37007) were transfected into cells using the siRNA Transfection Reagent (sc-29528, Santa Cruz Biotechnology) according to instructions supplied by the manufacturer.
Aliquots (5 μL) of individual PCR products were digested with two four-cutting restriction enzymes (HhaI and HaeIII ) for 16 hr according to instructions supplied by the manufacturer (Invitrogen).
Excess washing solution was removed, and the slides were incubated with 60 μl of ligation mixture in an open drop in a humidity chamber at 37 °C for 1 h, according to instructions supplied with the Duolink in situ Orange Starter Kit (Olink Biosciences).
