DNA polymerase Phusion (Finnzymes) was used in all amplification reactions according to manufacturer's instructions supplemented with DMSO and Betaine.
The human colorectal cancer cell lines Caco-2, DLD-1 and HCT 116 cells were routinely grown according to the supplier's instructions, supplemented with 10% fetal calf serum.
Ovular total RNA was extracted using the pBiozol Total RNA Extraction Reagent (BioFlux) according to the manufacturer's instructions, supplemented with two extra chloroform washes before nucleic acid precipitation.
This research utilizes a pre and post-test analytical model to compare an experimental, learner-centered approach to library instruction, supplemented with clickers, to a more traditional pedagogical approach.
Second, future comparative studies will benefit from a well-defined control that receives content instruction without a comic and a treatment group that receives the same instruction supplemented with an appropriate comic.
Strains harboring plasmids were grown in Pombe Minimal (PM) medium made from Edinburgh Minimal Medium (Bio-101), prepared according to the manufacturers instructions and supplemented with adenine at 75 µg/ml (PMA).
MCF10A and MCF10AT cells were maintained in complete HuMEC medium (per manufacturer instructions, Invitrogen) supplemented with 50 μg/mL streptomycin, and 50 U/mL penicillin.
Tumorigenic cell lines LNCaP CRL-17044), DU-145 (HTB-81), and PC-3 CRL-14355) and the non-tumorigenic cell line RWPE-1 (CRL-11609), and COS-7 cells (CRL-1651) were purchased from American Type Culture Collection (ATCC, Manassas, VA), cultured according to ATCC's instructions and supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin.
RWPE-1 (CRL-11609), RWPE-2 (CRL-11610), LNCaP CRL-17044), DU-145 (HTB-81), PC-3 (CRL-1435) and COS-7 CRL-16511) celinesnes were purchased from American Type Culture Collection (Manassas, VA), cultured according to ATCC's instructions and supplemented with 100 U/ml penicillin and 100 mg/ml streptomycin.
Both classes received conventional instruction; however, the experimental group's instruction was supplemented with the card game, while the control group's instruction was reinforced with traditional exercises and assignments.
Also, 7H11 (BD Difco) growth plate was prepared according to the manufacturer's instruction, and supplemented with 0.5% (v/v) glycerol, 0.05% (v/v) tween 80, 10% (v/v) OADC solution.
