Sentence examples for instructions started from inspiring English sources

Suddenly her coach's instructions started making sense.

Mussolini sent instructions: "Start violent action on Barcelona tonight, with constant hammering diluted over time".

Also, the instructions start with "Locate your breasts.

cDNA was synthesised according to the manufactures instructions starting with 2 µg RNA supplemented with 30 U M-MLV Reverse Transcriptase (Promega, Mannheim, Germany) and 0.625 µg 18-mer oligo dTs (Fermentas, St. Leon-Rot, Germany).

RNA isolation was performed according to the manufacturer's instructions, starting with washing with Buffer RW1.

Consecutive miRNeasy column purification was performed as per supplier instructions starting from step nine in the miRNeasy guidebook (Qiagen) [ 15].

RNA target for microarrays was prepared using the 3′ IVT Express Kit (Affymetrix, Santa Clara, CA) according to manufacturer's instructions starting with 200 ng of total RNA.

Illumina human oligonucleotide arrays (HumanHT-12) were used according to the manufacturer's instructions, starting with 750 ng of amplified RNA for each sample.

Probe preparation and hybridization to Genechip microarrays (Affymetrix; Santa Clara, CA) were performed according to Affymetrix instructions, starting with 6 μg of total RNA extracts.

Probe preparation and hybridization to Affymetrix Genechip® microarrays were performed according to Affymetrix instructions, starting with 6 μg of total RNA.

Biotinylated cRNA was prepared using the Illumina RNA Amplification Kit (Ambion) according to the manufacturer's instructions starting with from 200 ng total RNA from each sample.