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Reverse transcription and quantitative polymerase chain reaction (qPCR) were performed using 10 ng of RNA from each sample using SuperScript® III One-Step RT-PCR System (Invitrogen, Life Technologies) following manufacturer's instructions published elsewhere.
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Because detailed instructions of the methods employed have been published elsewhere [ 20], only a brief summary is presented here.
RNA was extracted from the worms stored in Trizol following the manufacturer's instructions and used to prepare a full-length enriched oligo-capped cDNA library in the pSPORT vector (Invitrogen) as published elsewhere [ 30].
However, studies are published elsewhere.
Detailed working conditions are published elsewhere [17].
The content has not been published elsewhere.
Further experimental details were published elsewhere [26].
The results will be published elsewhere.
This will be studied and published elsewhere.
The MRM-settings are published elsewhere [58].
More details will be published elsewhere.
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