BMSCs were cultured to passage 3 and plated in 6-well plates for β-galactosidase staining following the manufacturer's instructions provided with the β-Galactosidase Staining Kit (Cell Signaling Technology, Danvers, MA).
Credit card payments should be made up to the limit established by the credit card bank, in accordance with specific instructions provided with the invoices, to the Lockbox Bank designated for credit card payments.
Total RNA was extracted according to instructions provided with the utilized Trizol reagent (Invitrogen, Carlsbad, CA, USA); RNA concentration and content was evaluated via spectrophotometer, and cDNA was synthesized by reverse transcription of the RNA.
Saliva can be collected from adults by following the user instructions provided with the Oragene DNA self-collection kit.
DNA constructions were carried out by using established procedures [31] or following the instructions provided with specific reagents.
Chemiluminescence detection was performed according to the instructions provided with the Western Exposure Chemiluminescence Kit (GE Healthcare, USA).
Immuno-labelling using anti-glial fibrillary acidic protein (anti-GFAP) antibodies was performed according to the instructions provided with the Strept ABC Complex Kit.
TUNEL staining was done according to the instructions provided with the in situ cell death detection kit (Roche Diagnostics, Mannheim, Germany).
cAMP was measured according to the instructions provided with the EIA kit.
The ATP levels were measured and calculated according to the instructions provided with the BioThema kit.
Chemical crosslinking of antibodies was carried out following the manufacturer's instructions provided with the crosslinking reagents.
