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Also needed are free nucleotides used to build the new DNA strands and a DNA polymerase, an enzyme that does the building by sequentially adding on free nucleotides according to the instructions of the template.
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Follow the instructions of the guide.
Include instructions with the template.
RNA and DNA were extracted from plasma and PBMCs respectively according to the manufacturer's instructions of QIAamp as the template for a nested PCR [ 25, 28].
The genomic DNA of the strains from the collection was extracted with the YeaStar™ Genomic DNA kit (Zymo Research, Irvine, CA) following the instructions of the manufacturer, and used as a template to amplify deletion cassettes.
The gDNA of the strain IMS0344 was extracted with the YeaStar™ Genomic DNA kit (Zymo Research) following the instructions of the manufacturer, and used as a template to amplify by PCR the alleles rpn4-1 and rtg1-1.
You'll find step-by-step instructions for customizing the design right in the body of the template.
❖Definition of the template.
The first-strand cDNA synthesis, dC tailing and template switching and extension by RT were performed according to the instructions of the manufacturer.
Purified PCR products were used as templates for extension reactions with extension primers and iPLEX enzyme (iPLEX™ Gold Kit, SEQUENOM) following the instructions of the supplier.
Follow the instructions of the telephone representative.
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CEO of Professional Science Editing for Scientists @ prosciediting.com