Sentence examples for instructions of supplier from inspiring English sources

Plasmid preparation from E. coli strains was carried out using the Qiagen Plasmid Mini kit following the instructions of supplier.

LECs and HUVECs were purchased from Promocell (Heidelberg, Germany) and maintained per the instructions of the supplier.

Cellular cAMP levels were analyzed using the cAMP-Screen direct chemiluminescent competitive immunoassay system (Thermo Fisher Scientific), according to the instructions of the supplier.

Next, use a commercial MiniPrep plasmid isolation kit (e.g., MN NucleoBond) and follow the instructions of the supplier for high-copy plasmids.

To estimate the blood alcohol content (BAC) a commercial kit based on the alcohol dehydrogenase method (Hawkins et al. 1966) was used following the instructions of the supplier (SIGMA NAD-ADH Kit No 332-B. Sigma Chemical Company, St . Louis Mo).

Total RNA was extracted with the Trizol™ Reagent (Invitrogen) method following the instructions of the supplier.

The two-modules amplicon was cloned in pCR®4Blunt-TOPO® using the Zero Blunt® TOPO® PCR Cloning Kit for Sequencing (Invitrogen) following the instructions of the supplier.

PCR was performed using the Promega kit according to the instructions of the supplier.

Cells were transfected with JetPEI (QBiogen, Heidelberg, Germany) according to instructions of the supplier.

Purified PCR products were used as templates for extension reactions with extension primers and iPLEX enzyme (iPLEX™ Gold Kit, SEQUENOM) following the instructions of the supplier.

For single transfections, CHO cells were transfected with the cDNA corresponding to A2AR, A1R or D2R using lipofectamine (Invitrogen, Carlsbad, USA) method following the instructions of the supplier.