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Total DNA of SGC-7901, BGC-823, MGC803 and GES-1 cells was extracted according to the instructions of DNA extraction kit.
Leukocytes of the buffy coat suspended in 100 μl of aqueous were subjected for DNA extraction following the manufacturer's instructions of QIAGEN DNA extraction kit, Hilden, Germany.
Total RNA was extracted from cartilage (mirVana™ miRNA Isolation Kit Ambion, Life Technologies) and bone (miRNeasy Mini Kit, Quiagen) samples according to the instructions of the respective extraction kits.
The subcellular protein extraction followed the manufacturer's instructions of ProteoExtract Subcellular Proteome Extraction Kit (Calbiochem).
Nuclear and cytoplasm extractions of Min6 cells were performed according to the instructions of NE-PER NuCytoplasm Cytoplasm Extraction Reagents (Pierce Biotechnology, Rockford, IL, USA).
After visualisation, amplicons were extracted from the gel according to the instructions of the Qiagen Gel Extraction kit (Qiagen, Crawley, UK).
Cells (1 × 10) were harvested and lysates were extracted with 200 μl of extraction buffer following the manufacture's instruction.
Detailed instructions for data extraction and completion of the form should be prepared.
RNA was extracted via the manufacturers instructions for Trizol extraction.
We then added a further 180 µl of ATL and followed the manufacturer's instructions for DNA extraction.
Instructions of manufacturer was followed for the extraction of total RNA from treated and untreated cells using RNeasy protect bacteria mini Kit (catalogue no.74524, Qiagen,).
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