Sentence examples for instructions modified from inspiring English sources

cDNA was preamplified using the PreAmp® MasterMix Kit (ABI) according to the manufacturer's instructions, modified by employing half of the suggested volume of PreAmp reaction (25 μl PreAmp reaction volume).

First-strand cDNA was synthesized with 400 ng of total RNA from hOFs and hDFs or with 220 ng from hOFs-iPSCs using a WT Expression Kit (Affymetrix), according to the manufacturer's instructions, modified with additional ethanol precipitation.

53 57 The sample collection protocol and interpretation of test values was adapted from the IRIS and labeled urea manufacturer (http://www.helikit.com/en/physician-information/) instructions, modified according to the conclusions of the Gisbert and Parajes (2004) systematic review of validation studies.

As a result the Instrumar battery assembly instructions were modified to better contain the wires on new ELTs in April 2013, though not on those already in service.

Anti-human FOXP3 (clone 206D) was purchased from BioLegend (San Diego, CA) and intracellular staining performed with the FOXP3 staining kit according to manufacturer's instructions and modified as previously described [25].

PE-conjugated anti-Foxp3 (Ebiosciences) and intracellular staining was performed according to the manufacturer's instructions and modified as follows: 5×105 cells were stained for surface markers for 30 minutes at 22°C, fixed for 30 minutes with 1× Fix/Perm buffer.

Items and survey instructions were modified based on patient feedback.

Agents' rules may evolve, and they may copy their instructions when modified to represent growth.

The CSF cytokine levels were measured as per manufacturer's instructions and modified as previously described [ 20].

The concept maps of the pilot were left aside, because the instructions were modified after the pilot sessions.

The general instructions were modified to: "These questions collect information about how your injured knee affects you.