S.A.: "Pearls Before Swine," "Basic Instructions," "F Minus," and anything by Roz Chast.
Samples were thawed and resuspended in a denaturating solution (4 M guanidinium thiocyanate, 25 mM sodium citrate, pH 7, 0.5% sarcosyl) and extracted using RNeasy mini columns, according to manufacturer's instructions (RNeasy mini kit, Qiagen, Valencia, California).
We're always dealing with terror in retrospect – like stocking up on Imodium rather than reading the cooking instructions on your mini kievs.
DNA was extracted and purified from 3 ml of residual ThinPrep samples using a commercially available kit according to the manufacturer's instructions (QIAamp DNA mini kit, Tissue protocol, QIAGEN Inc, Valencia, Ca).
EMPTY THE MINI-BAR Years ago a friend who had recently gotten sober mentioned his habit of phoning hotels ahead with instructions to empty the mini-bar.
Following instructions on an iPad mini, with the world on the screen mirroring the environment built by the company, the audience move and silently interact in the exact place of the person whose story they're hearing.
Genomic DNA was isolated from macrophages of Cd36 +/+ and Cd36 -/- using column purification according to manufacturer' instructions (QIAamp DNA blood mini kit, Qiagen, Valencia, CA).
Total RNA was extracted following the manufacturer's instructions (Ambion; Purelink RNA mini kit) and purified using the Purelink DNase kit (Invitrogen).
Sedimented cells were ground in liquid nitrogen with a mortar and pestle and total RNA was extracted according to the manufactures instructions of RNeasy plant mini kit (Qiagen, Manchester, UK).
