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LightCycler 480 SYBR Green I Master kit (Roche Diagnostics) was used to perform 15 μl real-time RT-PCR reactions in 384-well plates according to the manufacturer's instructions (Light Cycler 480 real-time PCR system, Roche Diagnostics).
Read the instructions, light the flares and fill the tunnel with smoke.
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We used the interval from 50 ms before to 50 ms after the onset of the instruction (light pink shading in bottom panel of Figure 2A) to measure behavioral learning, and the simple-spike correlates of learning.
Read, the director of photography, had complied with Parrish's instructions to light the sequence in silhouette.
Alternatively, cytotoxicity was analysed using the ATPLite kit following the manufacturer's instructions, with light emission quantified using a BMG Labtech Fluostar plate reader.
Real time PCR was performed on a LightCycler® 480 II (Roche diagnostics, Mannheim, Germany) according to the manufacturer's instructions using light cycler mycobacterium detection assay.
Haas's score includes detailed instructions for lighting the performing space, so that the audience listens sometimes with the hall lit normally, sometimes in total darkness, while the music commutes between passages in normal tuning and those in microtones.
She was considering taking a self-defense class from a nearby karate studio that is offering free instruction in light of the slaying.
I divided it into two piles at either end of the grate, again in accordance with Mr. Guerra's instructions, and lighted them with a huge whoosh of accelerant.
Her early performance pieces included Lightning Piece from 1955, in which she sat at a piano and followed her own instruction to "Light a match and watch it till it goes out".
Some visually impaired are not totally blind and they can follow the instruction by light cue.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com