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Data on performance appraisal done by testing laboratory technologists based on the following characteristics in the check list: number of steps in the test procedure, clarity of kit instructions, kit and reagent packaging and labeling, and ease of performance, as used in the previous study, [ 10] were also obtained.
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Conventional curve analyses were performed according to the LightCycler instruction kit.
Many children today, he said, are too impatient to read instructions, so kit makers must keep them simple and include more pictures.
The mixture was processed by following the manufacturer's instructions of kit.
Sub-cellular protein fractions were isolated according to the manufacturer's instructions (ProteoExtract kit, EMD Biosciences, La Jolla, CA).
Immunostaining was performed as previously described on a Dako Autostainer per manufacturer's instructions (CSA kit, Dako) [49].
IFNγ concentration was determined by ELISA, according to the manufacturer's instructions (eBioscience kit, San Diego, CA).
The patients with TPO-Ab titer >12.0 IU/mL were considered positive according to instructions of kit manufacturer.
The reaction was revealed with enhanced chemiluminescence detection reagent according to the manufacturer's instructions (ECL kit; Amersham Pharmacia Biotech).
Immunohistochemical analysis of the TMA was carried out according to the manufacturer's instructions (Advance Kit, DAKO K4068, Glostrup, Denmark).
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