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Polyclonal goat α-mouse podocalyxin (1∶50, R&D Systems) and monoclonal mouse α-pan cytokeratin antibodies (1∶50, Sigma) were used according to the manufacturer's instructions in combination with the appropriate secondary antibodies.
TLSs were fixed in 10% formalin and stained using the Vectastain ABC Kit Vector Laboratoriess, USA), according to the manufacturer's instructions, in combination with rat anti-mouse CD31 (1 in 50 dilution) monoclonal antibody (BD Pharmingen, USA).
Two days later, T cell responses against the cognate peptides were analyzed by intracellular IFN-γ staining, using a cytokine staining kit (BD Pharmingen) according to the manufacturer's instructions in combination with goat anti-mouse CD4- and CD8-specific monoclonal antibody conjugates (BD Pharmingen).
Root canals were prepared using ProTaper instruments (Dentsply®, Konstanz, Germany) according to the manufacturer's instructions in combination with an Endo IT Professional (VDW®, Munich, Germany) at 250 rpm.
The supernatants were assayed in 10 mL samples for luciferase activity for 1 second using the Luciferase Reporter Assay system (Promega) according to the manufacturer's instructions, in combination with Wallac Victor 2 multilabel counter (PerkinElmer, Rodgau, Germany).
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More specifically, modeling and verbal instruction, in combination with a stepwise approach, were shown to be beneficial in the acquisition of procedural tasks.
Stanford system administrators implement SAML by way of modules and instructions, typically in combination with Shibboleth and Apache.
Total RNA from healthy specimens and 119 neoplastic specimens were extracted using Trizol® according to the manufacturer's instructions and in combination with Qiagen RNAeasy Mini Kit (Qiagen).
Spaced retrieval was offered in 11 studies used in combination with visual instructions, a stepwise approach, verbal instruction, and/or modeling.
This technique stressed speed and made use of many turning movements which, although already known, came under his instruction to be used more in combination.
Some studies have investigated the use of self-explanation in combination with direct instruction methods [ 65].
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