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In some experiments, 10T1/2 and TMNK-1 were stained with PKH67 and PKH26 dyes (Sigma Aldrich) according to the manufacturer's instructions for visualizing the morphology of the cells.
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Sucrose was removed using double distilled water and then developed in developing solution (National Diagnostics Atlanta, GA, USA) according to the instructions of the supplier for visualizing the staining.
Fractal approaches for visualizing huge hierarchies.
environment for visualizing images software.
Add StrepABComplex/horseradish peroxidase (DakoCytomation Glostrup, Denmark), prepared according to kit instructions, for 1 hr at RT. Immunoreactivity is visualized using Metal-Enhanced DAB (Pierce, Rockford, USA).
By following manufacturer's instruction, blots were visualized at the end by exposing membranes to autoradiography film for at least 5 minutes (MIDSCI, St . Louis MO).
See instructions for further details.
Tris-Acetate 3 8% gels were run, Western blotted, visualized and quantitated according to manufacturer's instructions for the NuPage system (Invitrogen) and Odyssey Infrared imaging system (Licor Biosciences).
See below for instructions for both cleaners.
Evidently, the general instructions for concept mapping as proposed by Novak [ 7] do not automatically lead to concept maps that visualize vertical integration.
Vectastatin kit was applied (Vector Laboratories) and staining was visualized by precipitation of Diaminobenzidine (DAB) (Sigma Aldrich) for nephrin or Vector SG substrate kit as per manufacturer's instructions for podocin.
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