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The students comprising the control group performed the experiments following the instructions described in the laboratory manual.
Total RNA was isolated from whole microdissected HFs using RNeasy Mini Kit (Quiagen) following the manufacturer's instructions described in the manufacturer's protocol.
The solution was stirred for 2 h at 0 °C and was then purified on a spin column following the instructions described in the EZ-link Sulfo-NHS-Biotinylation kit (ThermoFisher).
Hence to avoid this, the instructions described in the SOP must be understood.
Therefore, we consequently intended to guide the manual defining of the crescent-shaped VOI to further reduce the inter-reader variability using the instructions described in the "Methods" section.
Given the possible benefits of using these games to the generalization of reading and writing skills, it is important to emphasize that, different from some of the online and board games available for purchase, these games can be designed by anyone who can follow the design instructions described in the methods section.
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According to the manufacturer's instruction described in 'Materials and Methods', less than 25000 cells/40 μl/well were used in the 3D plates.
The fixed tissues were then embedded in paraffin, and TUNEL staining was performed according to the manufacturer's instructions as described in our previous studies29.
Capped RNA used for in vitro translation and RNA transfections was in vitro transcribed using the mMessage Machine kit (Ambion) per manufacturer's instructions as described in [16].
Also, concentrations of TGF-β1 and IGF-1 in the bladder protein samples were quantified using a commercially available immunoassay kit and following manufacturer's instructions, as described in [10].
All blood specimen collection and processing instructions are described in the KNHANES Laboratory/Medical Technologists Procedures Manual (13).
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