Print all arguments, following the order and formatting given in the INSTRUCTION column of the table above.
Each CyCustomGraphicsFactory informs the visual mapping mechanism of the chart type (e.g. lingrad) and method to create the CyCustomGraphics object given a String, which is the instruction column value.
The format of the instruction column is type: arglist, where type is the type of gradient or chart, and arglist is a list of name=value pairs that specify the arguments to create the gradient or chart (see details and examples in the tables below).
First digestion was performed according manufacturer's instructions then columns were washed with 350 µl of RW1 and digestion was repeated.
For purposes of microarray analysis and quantitative RT-PCR, total RNA was prepared using the miRNEasy kit (Qiagen, Valencia, CA) per manufacturer's instructions; on-column DNAse digestion was performed to eliminate contamination with genomic DNA.
Total RNA was extracted from cells using the Purelink Mini Total RNA Purification System (Invitrogen), according to the manufacturer's instructions for on-column DNAse treatment to remove contaminating DNA.
Total RNA was isolated from cell culture at day 0, 15, 15, 20, 30, 40, 50 and 60 of differentiation using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions with on-column DNase I digestion.
A loader template provides instructions where each column of the file should be stored in Chado.
RNA was extracted using the Plant RNA MiniPrepTM kit (ZYMO Research, Irvine, CA) according to manufacturer's instructions with on-column DNase treatment (Qiagen).
Total RNA was isolated from parasites with the High Pure Total RNA Isolation Kit (Roche) per the manufacturer's instructions, including on-column DNase I digestion.
