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FLAG-GNE transfected 293T cells (293T-FG) and untransfected (UT) control cells were grown in 75 cm2 flasks till sub-confluency (∼70%), crosslinked or not crosslinked with DSP (Pierce) according to the manufacturer instructions, collected and lysed as described above for muscle cells (without DTT and EDTA).
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When the participant is older than 6 years, awake, compliant, and capable of following instructions, collecting whole saliva by passive drool is optimal [ 17].
Mononuclear cells were isolated by Lymphoprep density centrifugation (Medinor, Oslo, Norway) according to the manufacturers' instructions and collected from the interphase layer.
We conducted live observations of physicians providing discharge instructions in the ED to 100 patients followed by a patient survey to determine their understanding in four domains (diagnosis, treatment plan, follow-up instructions, and return to ED (RTED) instructions) and collect patient demographics.
They were a set of instructions for collecting random ambient sound at a hundred and forty-seven locations, which were organized into groups of three to make the forty-nine "waltzes".
Following hours of interviews, as he recalled it, he was released, with instructions to collect documents about his criminal cases — the most recent of which was about nine years old.
He was recruited "several years" ago, the official said, with instructions to collect information about Bin Laden's network in the Federally Administered Tribal Areas, as his home region is officially known.
Controllers, in turn, make use of the Southbound API to interact with devices in the Forwarding plane, i.e., by issuing low-level instructions and collecting information.
After several weeks of productive botanising in Recherche Bay, in 1793 the expedition ships set sail for Tongatapu (main island in the kingdom of Tonga) where Delahaye had specific instructions to collect quality breadfruit plants for transport to the Isle de France.
All participants were given written and verbal instructions to collect a 24 h urine sample.
A second letter containing the FOBt kit and cardboard spatulas follows 1 2 weeks later with instructions for collecting the samples and a prepaid 'postage safe' return envelope.
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CEO of Professional Science Editing for Scientists @ prosciediting.com