RNA samples were isolated using Trizol® Reagent following manufacturer's instructions and cleaned using the RNeasy Mini kit (Qiagen, Hilden, Germany).
The RNA was isolated according to manufacturer's instructions, and cleaned with RNeasy clean-up kit (Qiagen, Valencia, CA, USA).
Full-length capped mbnl2 and MBNL2 mRNAs were synthesized using the mMessage mMachine kit (Ambion) according to the manufacturer's instructions, and cleaned up using Ambion's MEGAclear kit.
RNA was treated with Baseline-ZERO DNase (Epicentre, Madison, Wisconsin) according to the manufacturer's instructions then cleaned up with the Qiagen RNeasy Mini Kit and eluted in 32 µl of water.
Olineu, NIH3T3 or rat primary cells were homogenized in Trizol Reagent and RNA was isolated following manufacturer's instruction and cleaned using RNeasy Mini kit (Qiagen, Hilden, Germany).
C1 Banker's Last Day Testifying Frank P. Quattrone testified that if Credit Suisse lawyers "had told us that the document retention policy was suspended" he would not have endorsed a colleague's instructions to "clean up those files".
Anaya had asked to be billeted with a family with children her age; instead she was allocated to a childless couple, both of whom were 18 years old and who mostly ignored her for the duration of her stay, although they did leave explicit instructions to clean out their rabbit hutch.
Moreover, the staff has instructions to clean stethoscopes after each use.
RNA was treated with DNase I, RNase-free (Fermentas, Waltham, MA, USA) according to the manufacturer instructions and then cleaned using the Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA, USA).
DNA was extracted from stationary phase cultures of B. fragilis using the Promega Wizard Genomic DNA Purification Kit (as per manufacturer's instructions), and secondarily cleaned of residual RNA using Riboshredder (Epicenter, USA) and Zymoclean (Zymoresearch, USA) columns.
