Sentence examples for instructions by using the from inspiring English sources

[35S]-labelled proteins used for in vitro binding assays were produced by using the TNT Reticulocyte Lysate System or Wheat Germ System (Promega) according to the manufacturer's instructions, by using the corresponding pcDNA3 vectors as templates.

RNA was extracted according to the manufacturer's instructions by using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany).

Bound antibodies were revealed in accordance with the manufacturer's instructions by using the horseradish peroxidase developer 4-chloronaphthol (Bio-Rad).

All SNPs were genotyped with TaqMan Allelic Discrimination assays according to manufacturer's instructions by using the ABI PRISM 7000 sequence detector (Applied Biosystems, Foster City, CA).

Ligated DNA (5 μg) was randomly sheared by a nebulizer supplied with the Illumina paired-end sample preparation kit, then applied to paired-end sequencing as per the manufacturer's instructions by using the Illumina Genome Analyzer IIA.

Total RNA isolation from the RB primary tumor tissues (n = 30) was carried out as per manufacturer's instructions by using the miRCURY RNA Isolation Kit (cat. number 300111, Exiqon).

The validation of the results was performed according to the manufacturer's instructions and by using the provided positive and negative controls within the kit.

Genotyping of rs12979860 was performed using a 5′nuclease assay with allele-specific TaqMan probes, and assays were run on a 7900HT real time PCR instrument (Applied Biosystems, Carlsbad, CA, USA), following manufacturer's instruction or by using the tetra primer ARMS method [ 18, 19].

The data can either be entered into a spreadsheet, using the instructions provided, or by using the specifically developed Microsoft Access form-based entry tool.

4) Participants will receive instructions for self-monitoring by using the PEP forms and materials.

Cutoffs were set by using the instructions of the manufacturer.