Sentence examples for instructions as reported from inspiring English sources
The levels of the markers were measured in pre-treatment CSF using commercially available immunoassays (ELISA or multiplex bead suspension assay) following manufacturers' instructions as reported elsewhere [23].
Nine circulating cfnucleosome structures were measured using NuQ® ELISAs (Belgian Volition SA, Namur, Belgium) performed according to the manufacturer's instructions, as reported previously [ 19].
A Label Star array kit (Qiagen) was used for cDNA labelling according to the supplier's instructions as reported previously (Zhang et al, 2006).
An IFN‐ γ ELISPOT assay optimized to measure Lck‐specific CTL responses was performed according to the manufacturer's instructions as reported previously.
Epidermal growth factor receptor protein expression was evaluated on 3 μm thick tissue sections using the EGFR pharmDx assay (Dako Cytomation, Carpinteria, CA, USA) according to the manufacturer's instructions, as reported earlier (Frattini et al, 2007).
Cytokines were measured using a sandwich ELISA technique according to the manufacturer's instructions and as reported previously [30].
Cytokines were measured using a sandwich ELISA technique according to the manufacturer's instructions and as reported previously [ 13].
Insulin was measured using the rat insulin enzyme immunoassay kit (EIA), Spi-Bio, Montigny le Bretonneux, France, according to the manufacturer's instructions and as reported previously [ 25].
Online is a useful tool for doctors and patients, but it should be used only by those aware of its limitations, as advised in the program's instructions and as reported elsewhere.
The assessment of LOTCA test was performed according to instruction manuals as reported in the literature.
Bio-Plex assay (Bio-Rad, CA, USA, Cat No: M500KCAFoY) was utilised according to manufacturer's instructions and also as reported earlier [ 23] to measure the concentrations of 27 target proteins (Additional file 1: Table S2) using identical CRC plasma samples as used in the Proseek assay.
