Isolates were analysed following manufacturers instructions as previously described [26], with minor modifications.
Luciferase assays were performed by the Luciferase assay system (Promega) according to the manufacturer's instructions as previously described [12].
8-Plex iTRAQ (Applied Biosystems) sample preparation and labeling were performed according to manufacturer's instructions as previously described [23] with samples from Experiment 1 (Table 1).
NK cells were depleted by i.v. injection with 50 µl anti-ASIALO GM1 polyclonal antibody (Wako Chemicals, Osaka, Japan) or rabbit serum on day 22, 26 and 30, according to manufacturer's instructions as previously described [37].
Human peripheral blood monocytes (PBM) were isolated by centrifugation through a Ficoll gradient followed by CD14-positive Magnet-Assisted Cell Sorting (MACS, Miltenyi Biotec, Auburn, CA) according to manufacturer instructions as previously described [11].
Multiplex fluorescence in situ hybridisation (M-FISH) analysis was performed on metaphase spreads prepared from Res259 cells using a Vysis SpectraVysion probe (Abbot Molecular, Abbott Park, IL, USA) following the manufacturer's instructions as previously described [15].
Released cytokines TNFα and IL-6 and chemokines CXCL10 (IP-10), MIP-2α and IL-8 were quantified by DuoSet ELISA (R&D Systems, Minneapolis, MN) following the manufacturer's instructions as previously described [19].
A purified horseradish-peroxidase conjugated (HRP-conjugated) rat monoclonal antibody to galectin-3 (SPACE s.r.l., Milan, Italy) was used in immunohisto-cytochemistry according to the manufacturer's instructions as previously reported [13].
The test was performed according to the manufacturer's instructions, as previously described (2, 3 ).
The assay was performed according to the manufacturer's instructions as previously described [ 16, 27].
The detection procedure followed the manufacturer's instructions as previously described [ 12, 14- 17].
