In the course of the present study information about these instructions was obtained from participants.
When dosing instructions were missing from the pharmacy data, dosing instructions were obtained from the medical records.
Volumetric inspiratory CT and volumetric end-expiratory CT after standardized breathing instructions were obtained between July 2007 and September 2008.
Pre-instruction data were obtained for the introductory biology course, and pre- and post-data were obtained for the evolutionary biology course.
Pre- and post-instruction data were obtained for the evolutionary biology course (allowing us to address research questions 1, 2, and 3; N = 74).
The final instruction folders are available in online supplementary annexes 1 and 2. Written informed consent from the child's parent and the staff member performing the measurements in the attached instruction folders was obtained prior to photography.
Data regarding compliance with these follow-up instructions over time were obtained.
Immunodetection was carried out using the ECL western blotting detection system (GE Healthcare) according to the manufacturer's instructions and images were obtained using BIOMAX™-XBT X-ray film (Kodak).
Samples were run on Affymetrix U133a chips according to manufacturer's instructions, and data were obtained by using MAS5 parameters with global scaling set to 600.
Recombinant human cathepsins C (activated according to the manufacturer's instructions) and L were obtained from R&D Systems (Minneapolis, MN).
