He was a brilliant director, though: he gave the best instructions and was gentle and kind.
I dutifully followed the instructions and was surprised when some 15 seconds into the game, laughter began to ripple through the audience.
It was set up with no written or specific instructions and was located where it had little visibility and could not protect the security of the military manning it nor that of drivers of vehicles approaching it.
Few of these cases have been closed so far, but in one, in 1996, a jury awarded $16.5 million to the family of Brenda Young, a woman in Michigan, now 42, who was resuscitated in 1992 despite her written instructions, and was left with severe brain damage and little mental function, needing constant care.
Primary factor was calculated as per the instructions and was considered as personality type of that particular individual.
Total RNA was isolated using the Trizol reagent (Invitrogen) according to the manufacturer's instructions and was purified using the DNA-free RNA kit.
In other words, Minsky was convinced that computers could do so much more than execute a set of predefined instructions and was theorizing a model for a so-called artificial intelligence program.
Therefore extracellular DNA was quantified in the supernatant using a Quant-iT™ Picogreen® dsDNA Assay Kit (Invitrogen) according to the manufacturer's instructions and was analyzed with a SpectraFluor Plus absorbance reader (Tecan Group, Männedorf, Switzerland).
Total RNA was extracted from harvested synovial samples using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, and was used as a template for first-strand cDNA synthesis using SuperScript III RT (Invitrogen).
Total RNA was isolated from 30 mg tissue samples using the Nucleospin RNA II kit (Macherey Nagel, Germany) as per manufacturer's instructions and was treated with DNase I provided within the kit.
"He didn't obey instructions and was pulled over.
