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Nanny robots may one day be programmed with similar instructions and values.
Results were expressed in IU in accordance with the manufacturer's instructions, and values above 20 IU were considered positive.
Caspase 3/7 activity was measured using EnzChek Caspase-3 Assay Kit #2 (Invitrogen) according to the manufacturer's instructions and values adjusted for total cell number.
Luciferase activity was measured in extracts using the Luciferase Assay Reagent (Promega), according to the manufacturer's instructions, and values were expressed as relative light units normalized to the β-galactosidase activity or to the protein concentrations measured using the Bradford technique.
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His hope is that the people will carry with them these written words (which cover material they have already heard), both following his instructions and valuing the text as something to which they can keep returning (even though "we've heard this before").
"My master's degree work focused on behavior disorders, so I can say with confidence that it is not the children who are disordered," she writes noting that "the disorder is the system" -- which has inappropriate requirements, emphasizes disciplinary action over differentiating instruction, and values scores over meaningful teaching.
GADAs were analyzed by ELISA according to the manufacturer's instructions and the values reported as an index value in relation to standard serum, a level of above 10 IU/ml was regarded as positive according to the instructions in the kit; the method gives a maximum value of 250 IU/ml (15).
The threshold for analysis was set manually in the middle of the DNA control amplification curve, separately for each run, according to the manufacturer's instructions, and dCt values were obtained for (mutant target curve Ct) - (DNA control curve Ct).
Calculations for antibody titers were done according to the manufacturer's instructions and the values are reported as HU units/mL for IgG.
Each plasma sample was assayed according to the manufacturer's instructions, and the values were within the linear portion of the standard curve.
Expression of G6P and PEPCK was determined by real-time PCR on an ABI 7900HT Sequence Detection System (Applied Biosystems) according to the manufacturer's instructions, and target values were normalized to 18S rRNA.
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