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The difference between the sensory and the affective consequences of the painful stimulations were explained using standardized written instructions and using a number of practice trials.
Substrate phosphorylation was quantified using a Biosource Multiplex Bead Immunoassay kit (Invitrogen) according to the manufacturer's instructions and using a Luminex 100™ instrument (Luminex, Austin, TX).
The concentrations of BSA in the supernatants were determined with the BCA assay according to the supplier's instructions and using a multiscan GO spectrophotometer (Thermo Scientific, Finland).
First strand cDNA synthesis across the ligated mRNA ends was performed for cox1, cob, and cox3 using SuperScript III reverse transcriptase (Invitrogen) according to the manufacturer's instructions and using a gene specific primer designed for cDNA sequences (described above).
25(OH D was measured using an in vitro diagnostic enzyme immunoassay kit 25-Hydroxy Vitamin DS EIA (Immunodiagnostic Systems, Boldon, United Kingdom) according to the manufacturer's instructions and using a microtiter plate reader Spectramax 340PC (Molecular Devices, Sunnyvale, California, USA).
qRT-PCR for each transcript of interest was performed using TaqMan® One-Step RT-PCR Mander Mix ReagenTaqMan®and TaqMan® Gene Expression Assays on an ABI 7900HT Sequence Detection System (Applied Biosystems, Foster City, CA) according to manufacturer's instructions and using a 384 well plate format.
Similar(53)
The computer will help teachers to make media as a part of instruction and using a system of instruction, which is usually called as e learning in instruction.
Follow the instructions and use a 9x11 baking pan, unless otherwise indicated on the box.
Mix it with water according to the instructions and use a sponge.
This is healthy and normal.[7] If you do so, make sure to read all the instructions and use a disinfecting cleaner to keep toys clean and safe.[8].
TaqMan Copy Number Assays were performed according to the manufacturer's instructions (Applied Biosystems) and using an ABI7900HT instrument.
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