Sentence examples for instructions and using from inspiring English sources
Total microbial DNA was extracted using a MoBio PowerSoil DNA Extraction kit following manufacturer instructions and using 0.08 g of granule biomass.
Assays were done following manufacture's instructions and using as reference the expression levels of 18S.
The difference between the sensory and the affective consequences of the painful stimulations were explained using standardized written instructions and using a number of practice trials.
Substrate phosphorylation was quantified using a Biosource Multiplex Bead Immunoassay kit (Invitrogen) according to the manufacturer's instructions and using a Luminex 100™ instrument (Luminex, Austin, TX).
Luciferase assays were performed on cell lysates using Dual Luciferase assay kit (Promega), following the manufacturer's instructions, and using FLUOstar Omega luminometer (BMG Labtech).
The DNA sequences were determined in both senses using the Big dye Terminator v3.1 cycle sequencing kit (Applied Biosystems) according to the manufacturer's instructions and using the same primers as to PCR reactions.
Confronted with the RUF's horrific record and the imminent killing of large numbers of civilians, he defied his instructions and used UK troops to spearhead a counter attack.
The nuclear and cytoplasmic proteins were prepared using the Biovision Nuclear/Cytosol Fractionation Kit (Mountain View, CA) as per manufacturer's instructions and used in further analyses [43].
Cell-free zoster vaccine (Zostavax, Merck, Whitehouse Station, NJ) was reconstituted in sterile water following the manufacturer's instructions and used for some experiments.
Viruses were produced in HEK293 cells according to manufacturer's instructions and used to transduce cells, followed by selection in pre-determined concentration of puromycin.
The cellular suspension was diluted 13.64 times in IF-10 GN/GP (Biolog), dye G (Biolog) was added, according to the Biolog instructions, and used for plate inoculation.
