Sentence examples for instructions and used from inspiring English sources
Confronted with the RUF's horrific record and the imminent killing of large numbers of civilians, he defied his instructions and used UK troops to spearhead a counter attack.
After treatment with RNase-free DNase (TaKaRa, Shiga, Japan), mRNA was reverse transcribed using the PrimeScript RT Regent Kit (TaKaRa, Shiga, Japan) according to the manufacturer's instructions and used for analysis.
Genomic DNA was extracted from E. coli CVCC 1515 using a TIANamp Bacteria DNA Kit (Tiangen Biotech, Beijing, China) following the manufacturer's instructions and used as a PCR template.
The nuclear and cytoplasmic proteins were prepared using the Biovision Nuclear/Cytosol Fractionation Kit (Mountain View, CA) as per manufacturer's instructions and used in further analyses [43].
Viruses were produced in HEK293 cells according to manufacturer's instructions and used to transduce cells, followed by selection in pre-determined concentration of puromycin.
In the first experiment, PCR products of all diploid accessions were purified using ExoSAP-IT® (USB, Cleveland, USA) according to the manufacturer's instructions and used for direct sequencing.
Total microbial DNA was extracted using a MoBio PowerSoil DNA Extraction kit following manufacturer instructions and using 0.08 g of granule biomass.
Assays were done following manufacture's instructions and using as reference the expression levels of 18S.
The difference between the sensory and the affective consequences of the painful stimulations were explained using standardized written instructions and using a number of practice trials.
Substrate phosphorylation was quantified using a Biosource Multiplex Bead Immunoassay kit (Invitrogen) according to the manufacturer's instructions and using a Luminex 100™ instrument (Luminex, Austin, TX).
Luciferase assays were performed on cell lysates using Dual Luciferase assay kit (Promega), following the manufacturer's instructions, and using FLUOstar Omega luminometer (BMG Labtech).
