Sentence examples for instructions and subsequently from inspiring English sources
And if the phone is being used for navigation instructions and subsequently loses its cellular connection, the turn-by-turn alerts will also disappear.
On 30 November, she rendezvoused with Karanj at sea to transfer instructions and subsequently then left for Bombay and reached there by 4 December 1971.
For co-immunofluorescence to identify activated caspase 3 – positive and TUNEL – positive cells, slides were first TUNEL stained according to manufacturer's instructions and subsequently blocked and assayed with the activated caspase 3 antibody as above.
and 7-actinomycinD (7-AAD; Invitrogen) according to the manufacturer's instructions, and subsequently analysed by flow cytometry on a four laser LSR II SORP flow cytometer (BD Biosciences, San Jose, CA).
On the following day, myoblast cells were transfected with vinculin expression constructs using Nanofectin transfection reagent (PAA) according to the manufacturer's instructions and subsequently handled as detailed for MEFs (see above).
RIP and input RNA was treated with DNase I (Life Technologies) according to the manufacturer's instructions and subsequently ethanol precipitated.
Prior to analysis, we formatted the discriminating gene expression values from the test datasets onto spreadsheets according to software instruction, and subsequently applied the data to the software.
Amplicons obtained with the former primer set were cloned into the pENTR directional TOPO® vector according to the manufacturer's instructions (Invitrogen) and subsequently into the binary Gateway® 35S overexpression vector pK7WG2 [26].
Single-stranded phage DNA was prepared according to the Ph.D-12 phage display library manufacturer's instructions and was subsequently sequenced by Invitrogen Inc. (Shanghai, China).
Briefly, mRNA was enriched from 80~90 μg total RNA using the RNeasy Plant Mini Kit (QIAGEN) according to the instructions and was subsequently reverse-transcribed to double stranded cDNA using the GeneChip® Two-Cycle cDNA Synthesis Kit.
These might enabled them to grasp prior preparation instructions given by clinicians and subsequently help patients to comply with the instructions [ 19].
