Sentence examples for instructions and samples from inspiring English sources
Homogenized liver supernatant was prepared according to instructions, and samples were analyzed on a Bio-Plex Luminex 200 System at 635- and 532-nm wavelengths using Bio-Plex Manager 6.0 software.
Experiments were preformed according to the manufacturer's instructions and samples were run on BD LSR-II flow cytometer.
The final DNA sample was purified using a Qiagen MinElute PCR Purification Kit, following the manufacturer's instructions, and samples of the final product were assayed by quantitative real-time PCR using gene-specific primer sets (Table S1).
Extractions were performed according to manufacturers instructions, and samples were heated at 56°C for 1 h for protein digestion.
Total RNA was purified using Trizol reagent (Invitrogen Life Technologies, USA) according to the manufacturer's instructions and samples were analyzed as described previously [ 21].
The resulting dsDNA was purified using Illustra GFX DNA/gel clean-up kit (GE) as per manufacturer's instructions and samples eluted in 30 μl of nuclease-free water.
Master Mix was prepared as described in the instructions, and sample solutions were produced from 4 μl Master Mix and 1 μl DNA.
They found increased uptake of the two-sample FIT (39.6%) as compared with the three-sample FIT (23.4%), but it was unclear how the differences in test instructions and sampling techniques among brands may have affected completion.
Four hours later, both kidneys, liver, gut, and blood samples were collected, weighed, and submitted to lyses by SOLVABLE reagent (GNE9100, Packard Bioscience), according to the manufacturer's instructions, and sample radioactivity was determined using a beta counter (TRI-CARB 2100TR, liquid scintillation analyzer, Packard Bioscience).
The procedure was followed as described previously [30], following the manufacturer's instruction and samples were examined in triplicate for each condition.
CountBright absolute counting beads (Life Technologies) were added to allow an absolute count of the target cell population according to the manufacturer's instruction, and samples were analyzed immediately without any wash steps.
