Sentence examples for instructions and purified from inspiring English sources
IgG1 mAbs were grown in Celline flasks (IBS Integra Biosciences, Chur, Switzerland) according to manufacturer instructions and purified as follows.
Further, total RNA was isolated according to the TRIZol® manufacturer's instructions and purified with a QIAGEN® MiniElute® kit, following the protocol supplied by the manufacturer without modifications.
The RNA was DNase treated using RQ1 RNase free DNase (Promega) according to the manufacturer's instructions and purified using RNeasy Mini Kit (Qiagen) as recommended by the manufacturer.
Total RNA was isolated using the TRIzol reagent (Invitrogen) according to manufacturer's instructions, and purified with the RNeasy Mini kit (QIAGEN).
Total RNA was extracted from mice skins using Trizol (Invitrogen, Carlsbad, CA, USA) as the manufacturer's instructions and purified by RNAeasy Mini Kit (Qiagen, Valencia, CA, USA).
Probes were P-dCTP-labelled using Rediprime II Random Prime Labelling System (Amersham) according to manufacturer's instructions and purified with spin columns (Amersham).
P1 pAltermax plasmid was double-digested with EcoRI- XbaI (Fermentas), dephosphorylated with 10 U of Shrimp Alkaline Phosphatase (Fermentas) according to manufacturer's instruction, and purified by ethanol precipitation.
Total RNA was isolated using the Trizol reagent (Invitrogen) according to the manufacturer's instructions and was purified using the DNA-free RNA kit.
Total RNAs of leaves were extracted using TRIzol Reagent (Life technologies, USA) following the manufacturer's instructions, and further purified by RNeasy mini kit (QIAGEN, Germany) and RNase-Free DNase Set (QIAGEN).
Briefly, DNA was extracted from each mutant using QIAamp DNAmini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, and the purified DNA was then used as a template for a two rounds of nested PCR amplification.
Total RNA was isolated with Trizol reagent according to the manufacturer's instructions, and then purified with an RNeasy Kit.
