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All assessment assays and kits were performed in accordance with the manufacturers' instructions and protocols.
In Franz Kafka's The Trial 1n0ut found a world full of loops, repeats, instructions and protocols which are familiar parts of every computer programme.
Mutagenesis of levQ, levT and levS sequences was performed with the GPS®-LS linker-scanning (LS) system (New England Biolabs) according to the supplier's instructions and protocols described elsewhere [21].
Our research assistants were dedicated to establishing rapport with both GPs and reception staff, providing them with clear instructions and protocols, and 'fitting in' with their stated preferences.
When working with electricity, have first aid instructions and protocols handy.
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After RNA isolation for each sample, double stranded cDNA was synthesized from 500 ng total RNA and biotin-labeled using the GeneChip 3' IVT Express Kit (Affymetrix, Santa Clara, CA, USA, #901228-A) per the manufacturer's instructions and protocol.
Furthermore, the higher diversity of forest conditions (e.g., tree-species combinations – literally dozens in tropical forests - and various stand structural conditions), and the associated lack of simple, standardized instructions and management protocols make it more difficult to cope with rapid turnover of staff.
Specimens were tested by the eSensor XT-8 instrument according to the manufacturer's instructions and published protocols [ 6].
Essentially, exon trapping was performed using the exon trapping system formerly provided by Gibco/Life Technologies (now Invitrogen) according to the manufacturer's instructions and published protocols [ 22, 23] with minor modifications.
These research assistants were trained by the primary investigator on the use and administration of the EPAO tool, including discussing the tool's completion instructions and associated protocol.
A mouse-on-mouse IHC-ISO labeling kit (InnoGenex, San Ramon, CA) was used, according to the manufacturer's instructions and a protocol similar to one described (9 ).
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