We explicitly deemphasized reaction time in the instructions and measured performance by the percent correct trials (and their derivate false positives and hits), from the total of 96 trials comprising each task.
The amount of RNA extracted from tissue specimens was measured using the Quant-iT Ribo Green RNA assay (Invitrogen), adhering to the manufacturer's instructions, and measuring fluorescence for 0.1 seconds at excitations and emissions of 485 nm and 535 nm, respectively, using a Victor3 V 1420 Multilabel Counter (Perkin Elmer, Waltham, MA).
Cell apoptosis was analyzed using the Annexin V-FITC/PI Apoptosis Kit (Biosea, Beijing, China) according to the instructions and measured by flow cytometry.
Assays were performed according to manufacturer's instructions, and measured in Tecan GENios pro microplate spectrofluorometer (Tecan Group, Männedorf, Switzerland) with the Magellan V5.03 system (Tecan Group).
After 6 days incubation at 37°C and 95% humidity in 5% CO2 atmosphere, intracellular ATP was extracted using the CellTiter-GIo Luminescent Kit (Promega, USA) according to the manufacturer's instructions and measured by microplate luminometer (Berthod Diagnostic system, Germany).
For apoptosis detection after 48 h treatment, cells were stained with AnnexinV-APC/7-AAD (BD Pharmingen, Uppsala, Sweden) according to the manufacturer's instructions and measured by flow cytometry.
As the first federal effort since 1997 to examine instruction and measure student achievement in music and the arts, the survey has added new evidence to the debate about whether American schools are cutting back on the subjects they teach to concentrate on improving students' basic skills.
The adherent and floating cells were combined and treated according to the manufacturer's instruction and measured with FITC/PI staining using flow cytometry (Becton Dickinson, San Jose, CA).
On day 5, the stimulated cells were labeled by Brdu solution followed by developing the Eu-fluorescence as the manufacturer's instruction and measured the Eu-fluorescence counts using Victor2 1420 multilabel counter (PerkinElmer Life Sciences, Turku, Finland).
Patients are asked to measure and report their waist and hip circumference; instructions and a measuring device are provided.
