Sentence examples for instructions and libraries from inspiring English sources

Library construction was followed by Illumina sample preparation kit instructions, and libraries were sequenced on the Illumina Hiseq 2000 at Beijing Genome Institute.

Libraries were prepared for paired-end sequencing using the TruSeq™ RNA sample prep kits (Illumina, San Diego, CA) per manufacturer's instructions, and library size and integrity were determined using the Agilent Bioanalyzer 2100 (Santa Clara, CA).

Library preparation followed the manufacturer's instructions, and the libraries were pooled using adapter indices supplied by Illumina (barcodes AD012, AD013, AD014, AD015, AD016, and AD018).

as per instructions, and each library was constructed using a different Illumina barcoding primer.

Total RNA was extracted from mock treated (14 hpi) and B. cinerea infected (14 hpi) 4-week old plants (WT and wrky33) using the RNeasy Plant Mini kit (Qiagen) according to the manufacturer's instructions, and mRNA sequencing libraries were constructed with barcodes using the TrueSeq RNA Sample Preparation Kit (Illumina).

RNA was amplified using the ovation RNAseq system version 2 from Nugen according to manufacturer instructions and template DNA library construction was performed with the Encore Rapid Library System (Nugen).

To make matters worse, we have pushed Spanish as an alternative to English in commerce, on product instructions, in libraries, and worst of all, in schools, unlacing the bond of a common language.

After verifying that each sample presented a RIN number > = 7, Illumina RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation Kit, following the vendor instructions and the resulting cDNA libraries were sequenced using the Illumina Genome Analyzer IIx platform with a Paired End configuration of 144 cycles (72 bp read length pairs).

Each sample was prepared according to manufacturer's instructions and then the same library was run on two lanes of a Solexa/Illumina flow cell, resulting in two technical replicates for each biological replicate, runs were 36 base-pair paired end.

The implications of this study may inform curricular decisions by providing evidence-based instructional design for instructors wishing to employ situated learning in order to teach for transfer, thus improving distance education courses in general, and library instruction in particular.

Circular cDNA was then purified using AMPure XP beads (using 20 μl of the initial bead mixture, washed and resuspended in 2× sample volumes of PN buffer at ambient temperature, and proceeding with binding and washing as per the manufacturer's instructions) and the resultant bound cDNA library was eluted from the beads in 10 μl T10E0.1 buffer.