Transfection reagent used was Dharmafect following the product's instructions, and the experiments were performed three times in duplicate plates.
RASFs (2 × 10 cells in 100-mm-diameter dishes or 8.5 × 10 cells in 6-well plates) were transiently transfected with Sema4A small interfering RNA (siRNA, 50 nM, SI00133560, SI04174107, QIAGEN, Hilden, Germany) or negative control (QIAGEN, SI03650318) by Hiperfect transfection reagent (QIAGEN) following the manufacturer's instructions, and all experiments were performed 24 48 h after transfection.
In Experiment 1, participants spent more time viewing task-relevant information and less time viewing task-irrelevant information after instruction, and in Experiment 2, the presence of task-irrelevant information impaired performance.
Benefitting from a variety of supports and addressing several challenges, the teachers were able to advance their shared vision of curriculum and instruction at RSA, and experiment with a variety of approaches in an iterative attempt at achieving this vision.
Level of monitoring was reduced in the first experiment through test instructions and in the second experiment through speeded test responding.
RIF has been shown in retrieval-practice paradigm experiments, with exact instructions and procedures varying slightly from experiment to experiment.
HUVECs were transiently transfected with 1-5 μg plasmid DNA/106 cells with a Nucleofector kit VPB-10022) (Amaxa Biosystems, Cologne, Germany) according to the manufacturer's instructions, and used for experiments 24 h to 72 h after transfection.
Cells were transfected with the siRNA using Lipofectamine RNAiMAX (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions, and used for each experiment after 24 or 48 h.
Cell-free zoster vaccine (Zostavax, Merck, Whitehouse Station, NJ) was reconstituted in sterile water following the manufacturer's instructions and used for some experiments.
The conditions of reverse transcription and primer concentrations were set according to the recommendations in the manufacturer's instructions and confirmed by preliminary experiments which generated correlation coefficient (R) of each standard curve above 0.99.
