Sentence examples for instructions and detection from inspiring English sources
The PCR-reactions were performed according to the manufacturer's instructions and detection of the different genotypes made using an ABI PRISM® 7900HT Sequence Detector System (Applied Biosystems).
The PCRs were performed according to the manufacturers' instructions, and detection of the different genotypes was made using an ABI PRISM® 7900 HT Sequence Detector System (Applied Biosystems, Foster City, CA, USA).
DIG Wash and Block Buffer Set (Roche) was used for washing and blocking incubations according to manufacturer's instructions, and detection was made with CDP- Star (Roche) also following the instructions.
Sequencing reactions were carried out using the DyEnamic ET Dye Terminator Cycle Sequencing Kit (GE Healthcare, Pittsburg, PA, USA), with the primer −96 M13 (5'-OH CCC TCA TAG TTA GCG TAA CG-3') following the manufacturer's instructions, and detection was performed in a MegaBace 1000 Genetic Analyzer (Amersham Biosciences, Little Chalfont, UK) automatic capillary sequencer.
The immunoreactive bands were visualized and analyzed using the enhanced chemiluminescence detection reagents (Cell Signaling), according to the manufacturer's instructions, and chemiluminescence detection system (ChemiDoc XRS apparatus and software, Bio-Rad).
Caspase enzymatic activity was measured using the luminescence-based Caspase-Glo 3/7 Assay System (Promega, Madison, WI) according to the manufacturer's instructions and with signal detection on an Infinite M1000 microplate reader (Tecan, Morrisville, NC).
Detection and visualization of DNA was performed per the manufacturer's instructions (DIG DNA labeling and detection Kit, Roche).
Probe preparation, membrane hybridization and visualization were performed according to the manufacturer's instructions (DIG High Prime DNA Labeling and Detection Starter Kit II, Roche, Mannheim, Germany).
DNA fragments were transferred to the polyvinyl-based membrane using overnight capillary transfer followed by hybridization with digoxygenin (DIG -labeled CTX-M-15 FIA, anDIG -labeledon probes prepared according to the manufacturers instruction (DIG High Prime DNA labelling and Detection Starter Kit II, Roche, Germany) [ 5].
The gel was stained using SYPRO Ruby Life Technologiess) according to the manufacturer's instructions for detection and quantification of total protein content.
Cells treated with 25 ng/mL Emmprin or 100 µM deferoxamine (DFO) for 4 hours were immediately fixed; thereafter subjected to in situ PLA using Duolink Detection kit (Olink Bioscience, Uppsala, Sweden) according to the manufacturer's instructions for Duolink Blocking solution and Detection protocol.
