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Mononuclear cells were isolated by Lymphoprep density centrifugation (Medinor, Oslo, Norway) according to the manufacturers' instructions and collected from the interphase layer.
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Controllers, in turn, make use of the Southbound API to interact with devices in the Forwarding plane, i.e., by issuing low-level instructions and collecting information.
We conducted live observations of physicians providing discharge instructions in the ED to 100 patients followed by a patient survey to determine their understanding in four domains (diagnosis, treatment plan, follow-up instructions, and return to ED (RTED) instructions) and collect patient demographics.
The pre-publication history for this paper can be accessed here: http://www.biomedcentral.com/1471-230X/12/7/prepub The authors thank Prof. Gin-Ho Lo, M.D. for his guidance in treating some of the patients and to Mrs. Pi-Chin Wong for her assistance in scheduling the patients, providing instruction, and collecting data.
Cells were dissociated using Accutase digestion according to manufacturer's instructions (Innovative Cell Technologies, Inc., San Diego, CA) and collected by centrifugation.
Lin Yu provided the instruction of developmental biology and collected the datasets.
The professor intervened only as needed to help with the downloading, to clarify the written instructions, and to collect the completed exercise at the end of the class.
2, New Style), formally separated church from state, but it went far beyond its declared purpose by prohibiting religious bodies from engaging in instruction and from collecting dues from their members.
FLAG-GNE transfected 293T cells (293T-FG) and untransfected (UT) control cells were grown in 75 cm2 flasks till sub-confluency (∼70%), crosslinked or not crosslinked with DSP (Pierce) according to the manufacturer instructions, collected and lysed as described above for muscle cells (without DTT and EDTA).
The initial phase for performing sensing experiments consists of configuring the heterogeneous devices, sending them the instructions for starting the sensing and collecting the data.
For example, when children could not provide a urine sample, one center gave parents the supplies and instructions to collect the sample at home and arranged to pick it up the next day.
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