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The cDNA was synthesised by reverse transcription from 500 ng total RNA utilising the SuperScript First-Strand Synthesis System III for RT-PCR (Invitrogen, Darmstadt, Germany) according to the manufacturer's instructions and applying a combination of 50 ng random hexamer and 50 pmol oligo (dT 20 primers.
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"How are we going to take those instructions and apply it to our lives?" she said.
Start with a deck detergent — available at home centers and hardware stores — mix it according to the instructions and apply it with a garden-style sprayer, Mr. Lipford said.
The logical test measures capacity to understand written instructions and apply them to solving a problem.
The logical test measured capacity to understand written instructions and apply them to problem solving.
Control teams receive no specific SDM or ROM instructions and apply decision making as usual.
Briefly, the conditioned media was concentrated using Centricon (Millipore) according to the manufacturer's instructions and applied to 7.5% SDS polyacrylamide gels copolymerised with gelatine (0.1% w v−1) and incubated at 37°C for 24 h.
The PCR product was purified with ExoSAP-IT® (Amersham Biosciences, Uppsala, Sweden), labeled with DYEnamic ET Dye Terminator Cycle Sequencing Kit for MegaBACE DNA Analysis Systems™ (Amersham Biosciences), following the manufacturer's instructions, and applied to MegaBACE DNA Analysis Systems™ (Amersham Biosciences).
Quantitative real-time PCR was performed for cDNA amplification using SYBR Premix ExTaq (Takara Bio, Inc., Shiga, Japan) and primers listed in Table 1, on a Bio-Rad C1000 real-time system (Bio-Rad, Hercules, CA, USA), according to the manufacturer's instructions and applied international standards.
Allow the clear coating to dry per the manufacturer's instructions, and apply as many coats as desired.
Take your time applying the materials, as you will most likely only have one chance to apply them.Make sure you read the instructions and apply thoroughly, to avoid the materials falling off.
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