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For the isolation and collection of surface proteins, we used the Pierce Cell Surface Protein Isolation Kit (ThermoScientific, Waltham, MA, USA), following the manufacturer's instructions adjusted according to the protocol described by De Wit et al (2012); Supplementary File S1 provides an elaborate description of the cell surface protein isolation and mass spectrometry protocol.
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ATP concentrations in the lysates were quantified in triplicate using an ATP determination kit (luciferase-luciferin; Life Technologies) in a BioTek FL600 microplate reader according to the manufacturer's instructions (results were adjusted according to the protein concentration).
The working parameters of arsenic-hollow cathode lamp were adjusted according to the manufacturer instructions.
Type1 rat-tail collagen (3.69 mg/mL; BD Bioscience, Bedford, MA) was adjusted according to the manufacturer's instructions.
The parameters of ablation, including ablation power, ablation time, and number of electrodes, were adjusted according to the manufacturer's instructions, operator's experience, and intraoperative CT evaluation.
The path length for NIRS measurements was adjusted according to the manufacturer's instructions for measurements on the adult human skull and sampling rate was set to 6 Hz.
Students enrolled in classes which begin before the official "Instruction Begins" date on the academic calendar will have their refund percentages adjusted according to the day ranges listed after the date ranges.
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