The MCF-10A, MDA-MB-231, MDA-MB-361, BT-474 and T-47D cell lines were purchased from ATCC (Manassas, VA, USA) and cultured according to ATCC instructions, except for MCF-10A, which was maintained as previously described [ 17].
Rater 'masking' was maintained by providing specific instructions to participants and their clinical teams not to disclose treatment details.
HepG2 was maintained in MEM with supplements according to ATCC cell culture instructions.
Flag-VAPB and Flag-FAF1 U2OS cells were generated from Flp-In T-REx U2OS cells, following Invitrogen's instructions and were maintained in McCoy's media supplemented with 10% FBS, 2 mM L-glutamine, 100 units/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml hygromycin, 15 μg/ml blasticidin (Invitrogen).
A display in the cab reproduces either the aspects of signals ahead or up to 10 different instructions of speed to be maintained, decelerated to, or accelerated to, according to the state of the track ahead.
The instructions also say that "appropriate buffer zones must be maintained" to avoid contaminating surrounding areas.
Candida albicans (MTCC227), Candida krusei (MTCC9215) were obtained from Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh and the cultures were maintained as per the MTCC instructions.
22RW1 cells were maintained according to manufacturer's instructions (ATCC) and proteins were extracted as previously described [18].
Zebrafish were maintained in recirculating tanks following instructions from ' The zebrafish book' (Westerfield, 2000).
The three cell lines were maintained according to the manufacturer's instruction.
